Journal: Cell Structure and Function

Article Title: Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells

doi: 10.1247/csf.22074

Figure Lengend Snippet: Optogenetic manipulation of gene expression in hepatocytes by HTVi and noninvasive blue light irradiation (A) Photograph of blue light-irradiated mouse after hydrodynamic tail vein injection (HTVi). The abdomen was depilated and irradiated with blue light using an LED array. (B) Time course of the experiment and light irradiation condition. Mice were exposed to blue light for 20 h at 90.0 mW cm –2 (1 min on and 1 min off cycles). (C) The mouse hepatocytes were transfected with eGAV expression vector, 5x UAS-Achilles-NLS-PEST-Hes1 3' UTR reporter, and mCherry-NLS expression vector as a transfection marker. (D–F) Representative images of gene expression induced by blue light irradiation in eGAV-transfected mouse liver (D, E) and hGAVPO-transfected mouse liver (F). White boxes in D were enlarged in E. Higher leakage expression of Achilles was observed in hGAVPO-transfected livers than eGAV-transfected livers. Experiments were repeated three times ( n = 3). Scale bars, 2 mm for (D) and 500 μm for (E, F).

Article Snippet: Luminescence signals at the population level were recorded by a live cell monitoring system (Churitsu Electric Corp., Nagoya, Japan, CL24B-LIC/B) equipped with a highly sensitive photomultiplier tube (PMT) and an LED blue light source (OptoCode, LEDB-SBOXH).

Techniques: Gene Expression, Irradiation, Injection, Transfection, Expressing, Plasmid Preparation, Marker